abx mixture Search Results


antibiotic mixture abx  (Jackson Laboratory)
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Jackson Laboratory antibiotic mixture abx
Extended <t>ABX</t> treatment alters IgG subclass distribution during homeostasis. (A) CFUs grown on LB agar and BHI agar plates from fecal bacteria <t>after</t> <t>antibiotic</t> treatment of mice (n = 5 mice/group). (B) Stool samples sequenced using 16S data and analyzed to determine phylum level relative abundance. Serum Ig titers of WT (white filled) and ABX-treated mice (black filled) for (C) mIgG1 (n = 20–30), (D) mIgG2a/c (n = 20–30), (E) mIgG2b (n = 20–30), (F) mIgG3 (n = 20–30), (G) mIgE (n = 20–30), (H) mIgM (n = 10), (I) mIgA (n = 20), and (J) fecal mIgA (n = 10) determined by ELISA. Means and SEM (±SEM) are plotted. Results are representative of at least two independent repeats. The p values are for two-tailed unpaired Student t test were performed for statistical comparisons. *p < 0.05, **p < 0.01, ****p < 0.001. ns, not significant.
Antibiotic Mixture Abx, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Extended ABX treatment alters IgG subclass distribution during homeostasis. (A) CFUs grown on LB agar and BHI agar plates from fecal bacteria after antibiotic treatment of mice (n = 5 mice/group). (B) Stool samples sequenced using 16S data and analyzed to determine phylum level relative abundance. Serum Ig titers of WT (white filled) and ABX-treated mice (black filled) for (C) mIgG1 (n = 20–30), (D) mIgG2a/c (n = 20–30), (E) mIgG2b (n = 20–30), (F) mIgG3 (n = 20–30), (G) mIgE (n = 20–30), (H) mIgM (n = 10), (I) mIgA (n = 20), and (J) fecal mIgA (n = 10) determined by ELISA. Means and SEM (±SEM) are plotted. Results are representative of at least two independent repeats. The p values are for two-tailed unpaired Student t test were performed for statistical comparisons. *p < 0.05, **p < 0.01, ****p < 0.001. ns, not significant.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Modulating T follicular cells in vivo enhances antigen-specific humoral immunity

doi: 10.4049/jimmunol.2001434

Figure Lengend Snippet: Extended ABX treatment alters IgG subclass distribution during homeostasis. (A) CFUs grown on LB agar and BHI agar plates from fecal bacteria after antibiotic treatment of mice (n = 5 mice/group). (B) Stool samples sequenced using 16S data and analyzed to determine phylum level relative abundance. Serum Ig titers of WT (white filled) and ABX-treated mice (black filled) for (C) mIgG1 (n = 20–30), (D) mIgG2a/c (n = 20–30), (E) mIgG2b (n = 20–30), (F) mIgG3 (n = 20–30), (G) mIgE (n = 20–30), (H) mIgM (n = 10), (I) mIgA (n = 20), and (J) fecal mIgA (n = 10) determined by ELISA. Means and SEM (±SEM) are plotted. Results are representative of at least two independent repeats. The p values are for two-tailed unpaired Student t test were performed for statistical comparisons. *p < 0.05, **p < 0.01, ****p < 0.001. ns, not significant.

Article Snippet: The 5–6-wk-old C57BL/6 mice for antibiotic mixture (ABX) experiments and 7–8-wk-old C57BL/6 and NOD mice were purchased from The Jackson Laboratory and maintained in the animal facility at Massachusetts General Hospital under specific pathogen-free conditions according to the National Institutes of Health guidelines.

Techniques: Bacteria, Enzyme-linked Immunosorbent Assay, Two Tailed Test